Second round completed in Miami.

2025 Miami Open presented by Itaú, a WTA 1000 tournament, on Hard Outdoor, Miami, Florida, USA, 18-30 March 2025.

Defending champion:

Danielle Collins who defeated Elena Rybakina (4) 7-5 6-3

Second round results:

- Emma Raducanu defeated Emma Navarro (8) 7-6(6) 2-6 7-6(3)

- McCartney Kessler defeated Linda Noskova (30) 2-6 7-5 7-6(4)

- Amanda Anisimova (17) defeated Mayar Sherif 6-2 6-2

- Mirra Andreeva (11) defeated Veronika Kudermetova 6-0 6-2

- Anna Blinkova defeated Diana Shnaider (13) 6-4 7-6(0)

- Marta Kostyuk (23) defeated qualifier Kimberly Birrell 7-5 4-6 6-3

- Anna Kalinskaya (32) defeated Moyuka Uchijima 6-1 6-3

- Jessica Pegula (4) defeated qualifier Bernarda Pera 6-4 6-4

- Madison Keys (5) defeated Elina Avanesyan 6-3 6-3

- Wildcard Alexandra Eala defeated Jelena Ostapenko (25) 7-6(2) 7-5

- Clara Tauson (20) defeated Julia Grabher 6-4 7-5

- Paula Badosa (10) defeated wildcard Victoria Mboko 7-5 1-6 7-6(3)

- Karolina Muchova (15) defeated Victoria Azarenka 6-0 (retired)

- Elina Svitolina (22) defeated Belinda Bencic 6-1 6-2

- Elise Mertens (27) defeated Peyton Stearns 6-4 6-1

- Iga Swiatek (2) defeated Caroline Garcia 6-2 7-5