AI Boom Continues: 49 US Startups Secure Over $100M Funding in 2025

The year 2025 has solidified its position as a monumental period for the artificial intelligence industry, particularly within the United States, showcasing a significant acceleration in investment compared to the already robust trends of 2024. TechCrunch's analysis reveals that while 2024 saw 49 AI startups raising funding rounds of $100 million or more, with three companies securing multiple “mega-rounds” and seven reaching $1 billion or more, 2025 has already matched or exceeded these figures. Crucially, 2025 has also witnessed a substantially higher number of companies successfully closing multiple funding rounds greater than $100 million, indicating a heightened and sustained investor appetite for AI innovation across diverse sectors.
Investment activity throughout 2025 has demonstrated consistent strength, fueling companies engaged in foundational models, core AI infrastructure, and specialized applications across critical domains such as healthcare, legal technology, and enterprise software. The sheer volume and scale of these funding rounds underscore a profound investor confidence in the transformative capabilities of AI technologies and their long-term market potential.
November brought several high-profile capital infusions into the AI landscape. Anysphere, the developer of the popular vibe coding platform Cursor, secured an enormous $2.3 billion funding round, elevating its valuation to $29.3 billion. This marked the company's second substantial funding round within the year. Parallel, focusing on the development of web infrastructure for AI agents, successfully raised a $100 million Series A round, co-led by prominent venture capital firms Index Ventures and Kleiner Perkins. Hippocratic AI, a startup specializing in healthcare AI agents, closed its second funding round of the year with a $126 million Series C, which propelled its valuation to $3.5 billion, with Avenir Growth leading the investment.
October continued the strong momentum with numerous significant investment announcements. Fireworks AI, a platform empowering users to build AI applications using open-source models, raised $250 million in a Series C round, achieving a $4 billion valuation. Enterprise AI firm Uniphore secured $260 million in a Series F round, valuing the company at $2.5 billion, with notable participants including Snowflake Ventures, Nvidia, Databricks Ventures, and AMD. Voice AI company Sesame raised a $250 million Series B round, co-led by Sequoia and Spark Capital. Cambridge, Massachusetts-based OpenEvidence, which develops an AI chatbot for the medical field, received its second 2025 funding with a $200 million Series C, valuing it at $6 billion. Lila Sciences, dedicated to building a science superintelligence platform, announced its second funding round of 2025, a $350 million Series A, co-led by Braidwell and Collective Global. DeepSeek competitor Reflection AI announced its second mega-round of the year, a $2 billion Series B round led by Nvidia, valuing the company at $8 billion. EvenUp, which applies AI to the personal injury legal sector, announced a $150 million Series E round, valuing it at more than $2 billion.
September proved to be an exceptionally active month, featuring a high concentration of significant capital raises. Periodic Labs, actively building an AI scientist, announced a $300 million seed round led by Felicis and Andreessen Horowitz, with additional participation from Nvidia. AI infrastructure company Cerebras Systems raised a substantial $1.1 billion Series G round, valuing it at $8.1 billion, co-led by Fidelity and Atreides Management. Modular secured $250 million in funding, led by the US Innovative Technology Fund. Distyl AI, specializing in enterprise AI software, raised a $175 million Series B round, valuing the startup at $1.8 billion. Upscale AI, another promising AI infrastructure startup, secured a $100 million seed round. Groq, an AI inference company, raised a $750 million Series E round, valuing it at nearly $6.9 billion. Invisible Technologies, an AI training startup, reached a $2 billion valuation after a $100 million fundraise. Cognition AI, the innovator behind the Devin coding agent, raised a $400 million Series C round, valuing the company at $10.2 billion. AI infrastructure firm Baseten secured a $150 million Series D round, valuing it at $2.1 billion. Bret Taylor’s customer service AI agent platform Sierra raised $350 million, valuing it at more than $10 billion. Personalized AI search engine You.com raised a $100 million Series C round, valuing the company at $1.5 billion. AI research lab Anthropic announced its second funding round of 2025, a colossal $13 billion Series F, which propelled the company's valuation to an astounding $183 billion, led by Iconiq, Fidelity, and Lightspeed.
August saw EliseAI, a platform for healthcare and housing automation, raise $250 million in a Series E round, valuing the startup at $2.2 billion. AI research lab Decart also secured $100 million at a $3.1 billion valuation.
In July, generative media platform Fal successfully raised a $125 million Series C round, valuing the company at $1.5 billion. Ambience Healthcare, focused on building an AI healthcare operating system, raised $243 million in a Series C round. AI research lab Reka AI secured $110 million in funding, achieving a $1 billion valuation. Thinking Machines Lab confirmed a $2 billion seed round, led by Andreessen Horowitz with participation from Nvidia, valuing it at $12 billion. Cambridge, Massachusetts-based OpenEvidence, an AI-powered search tool for clinicians, raised $210 million, valuing it at $3.5 billion. Harmonic, which is developing a mathematical reasoning engine, raised a $100 million Series B round.
June's notable funding rounds included healthcare AI unicorn Abridge, which announced its second round of 2025 with a $300 million Series E, valuing the company at $5.3 billion. Harvey, a provider of AI tools for the legal industry, also announced its second $300 million round of 2025, a Series E, which brought its valuation to $5 billion. Healthcare AI startup Tennr raised a $101 million Series C round. Enterprise search startup Glean continued its impressive fundraising trajectory, securing a $150 million Series F round, valuing it at $7.25 billion. Anysphere, the AI research lab behind the AI coding tool Cursor, raised a sizable $900 million Series C round, valuing the company at nearly $10 billion.
May witnessed AI data labeling startup Snorkel AI announcing a $100 million Series D round, valuing the company at $1.3 billion. LMArena, a community-driven benchmarking tool for AI models, raised a $100 million seed round, valuing the startup at $600 million. Las Vegas-based AI infrastructure company TensorWave announced a $100 million Series A round.
April featured SandboxAQ closing a $450 million Series E round, valuing the AI model company at $5.7 billion. Runway, which creates AI models for media production, raised a $308 million Series D round, valuing the company at $3 billion.
March was significantly highlighted by AI behemoth OpenAI's record-breaking $40 billion funding round, which valued the startup at an astounding $300 billion, led by SoftBank. Nexthop AI, an AI infrastructure company, announced a $110 million Series A round. Insilico Medicine raised $110 million for its generative AI-powered drug discovery platform, valuing it at $1 billion. Celestial AI, an AI infrastructure company, raised a $250 million Series C round, valuing the company at $2.5 billion. Lila Sciences secured a $200 million seed round as it aims to create a science superintelligence platform. Brooklyn-based Reflection.Ai, focused on building superintelligent autonomous systems, raised a $130 million Series A round, valuing the one-year-old company at $580 million. AI coding startup Turing closed a $111 million Series E round, valuing the startup at $2.2 billion. Shield AI, an AI defense tech startup, raised $240 million in a Series F round, valuing the company at $5.3 billion. AI research and large language model company Anthropic raised $3.5 billion in a Series E round, valuing the startup at $61.5 billion.
February saw Together AI, a creator of open-source generative AI and AI model development infrastructure, raise a $305 million Series B round, valuing the company at $3.3 billion. AI infrastructure company Lambda raised a $480 million Series D round, valuing the startup at nearly $2.5 billion. Abridge, an AI platform that transcribes patient-clinician conversations, was valued at $2.75 billion after a $250 million Series D round. Eudia, an AI legal tech company, raised $105 million in a Series A round. AI hardware startup EnCharge AI raised a $100 million Series B round. Harvey, another AI legal tech company, raised a $300 million Series D round, valuing the three-year-old company at $3 billion.
Finally, January commenced the year with synthetic voice startup ElevenLabs raising a $180 million Series C round, valuing the company at more than $3 billion. Hippocratic AI, dedicated to developing large language models for the healthcare industry, announced a $141 million Series B round, valuing the company at more than $1.6 billion. This consistent and robust flow of significant investments, spanning various funding stages and diverse applications, unequivocally highlights the AI industry's ongoing rapid expansion and its pivotal role in shaping future technological landscapes.
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